Comparison of Human Erythrocyte Filterability with Trapping Rate Obtained by Nickel Mesh Filtration Technique: Two Independent Parameters of Erythrocyte Deformability

Main Article Content

Takeshi Arita
Mitsuhiro Fukata
Toru Maruyama
Keita Odashiro
Takehiko Fujino
Chizuko Wakana
Aya Sato
Kazue Takahashi
Yoshiko Iida
Shiro Mawatari
Koichi Akashi

Abstract

Background: Erythrocyte deformability is a major determinant of microcirculation in vivo. Although this concept was defined as filterability evaluated by flow-pressure curve constructed during a filtration process of erythrocyte suspension through a nickel mesh filter, the behaviours of erythrocytes during the filtration process are unknown.

Aim: The present study aimed to obtain the better rheological understanding of the behaviours of erythrocytes passing through the nickel mesh filter.

Methods: Blood sample was obtained from 8 apparently healthy subjects after obtaining informed consent. Erythrocyte filterability (%) was calculated as the flow rate of a hematocrit-adjusted erythrocyte suspension relative to that of saline at a filtration pressure of 100 mmH2O in flow-pressure curves obtained by nickel mesh filtration technique. Nickel mesh filters showing specific pore sizes of 6.00 μm (step 1) and 5.31 μm (step 2) were chosen, and two-step filtration protocol was performed. Erythrocytes counts (EC) were performed immediately before (ECpre) and after (ECpost) each filtration, and erythrocyte trapping rate (%) was defined as (ECpre – ECpost) / ECpre. Erythrocyte filterability and trapping rate were correlated in each step for data analysis.

Results: ECpre was always greater than corresponding ECpost in every subject and in both steps. Erythrocyte filterability in the step 1 (91.8 ± 2.1%) was close to that in the step 2 (90.0 ± 10.3%). Likely, the trapping rate in the step 1 (77.8 ± 2.4%) was close to that in the step 2 (79.4 ± 7.0%). Mean filterability in step 1 did not differ from that in step 2 (p = 0.637), and the same was true with respect to the mean trapping rate (p = 0.516). However, individual comparison between the filterability and the trapping rate of both steps demonstrated no correlation.

Conclusions: The present findings indicate that erythrocytes in suspension are trapped substantially by our nickel mesh filter. This trapping rate was not correlated to the erythrocyte filterability obtained by the flow-pressure curve during the nickel mesh filtration. Therefore, the erythrocyte trapping rate should be considered as a hemorheological parameter independent of the erythrocyte filterability.

 

Keywords:
Deformability, erythrocytes, filtration, nickel mesh, trapping

Article Details

How to Cite
Arita, T., Fukata, M., Maruyama, T., Odashiro, K., Fujino, T., Wakana, C., Sato, A., Takahashi, K., Iida, Y., Mawatari, S., & Akashi, K. (2018). Comparison of Human Erythrocyte Filterability with Trapping Rate Obtained by Nickel Mesh Filtration Technique: Two Independent Parameters of Erythrocyte Deformability. International Blood Research & Reviews, 8(4), 1-8. https://doi.org/10.9734/IBRR/2018/44667
Section
Original Research Article