Haematological and Hepatorenal Alterations Induced by Potash (Akanwu) on Male Wistar Rats

Bruno Chukwuemeka Chinko *

Department of Human Physiology, Faculty of Basic Medical Sciences, College of Health Sciences, University of Port Harcourt, Port Harcourt, Rivers State, Nigeria.

Dibo Tabot Pughikumo

Department of Human Physiology, Faculty of Basic Medical Sciences, College of Health Sciences, Niger Delta University, Wilberforce Island, Amassoma, Bayelsa State, Nigeria.

*Author to whom correspondence should be addressed.


Abstract

Introduction: Potash refers to the various naturally occurring water-soluble compounds of potassium used as a traditional food additive in Nigeria. Several health warnings have highlighted the deleterious effect of their consumption.

Aim: The present study is aimed at evaluating the effects of graded doses of potash consumption on haematological and hepatorenal indices using Wistar rat models.

Materials and Methods: Potash was locally sourced and the elemental composition was determined by X-Ray diffraction (XRD). Twenty (20) male Wistar rats (170 – 200g) were used for the study and were divided into four (4) groups of five (5) animals each. Group 1 served as the control while Groups 2 – 4 served as the experimental group and received an aqueous mixture of potash via oral gavage at 200, 400 and 800mg/kg respectively for thirty (30) days.

Results: XRD elemental analysis of potash revealed the presence of potassium (27.44%), phosphorus (14.27%), antimony (5.18%), barium (4.27%), fluorine (2.78%), cadmium (2.06%), yttrium (1.63%), tellurium (1.32%), caesium (1.12%), niobium (1.04%), sodium (0.42%) and germanium (0.06%). Results from haematological and hepatorenal parameters show a significant reduction in mean values of red blood cell count,  haemoglobin concentration and packed cell volume among the experimental groups compared to control (p<0.05) and significantly increased mean corpuscular volume, mean corpuscular haemoglobin, white blood cell count, lymphocytes, neutrophils, MID cell percentage, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, urea, creatine, sodium, potassium and chloride ions among the experimental group compared to the control (P<0.05).

Conclusion: The data presented has shown that the chronic consumption of potash at 800mg/kg can negatively alter the haematological and hepatorenal profiles of Wistar rats. Therefore, this study considers potash, a possible toxicant to the blood, liver and kidney.

Keywords: Potash, akanwu, haematology, hepatorenal, liver and kidney


How to Cite

Chinko, B. C., & Pughikumo, D. T. (2023). Haematological and Hepatorenal Alterations Induced by Potash (Akanwu) on Male Wistar Rats. International Blood Research & Reviews, 14(1), 38–46. https://doi.org/10.9734/ibrr/2023/v14i1300

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References

Garrett DE. Potash: deposits, processing, properties and uses: Springer Science & Business Media; 2012.

Airaodion AI, Emaleku SA, Osunmuyiwa OJ, Megwas AU, Ayita EB, Oluba SO, et al. Nephrotoxic nature of potash (Kaun) in wistar rats. International Journal of Health, Safety and Environment. 2021; 7(04):830-7.

Warren JK. Evaporites through time: Tectonic, climatic and eustatic controls in marine and nonmarine deposits. Earth-Science Reviews. 2010;98(3-4):217-68.

Khan IA. Management of Agricultural Inputs. Rajasthan: Agrotech Publishing Academy; 2014.

Momoh TB, Yaro CA, Usuman SO, Iyeh VA. Effect of potash on the tenderness and phytochemical constituents of Cajanus Cajan. Trends Applied Sciences Research. 2019;14(4):278-82.

Kutshik RJ, Idogun FO, Ujah FE, Gotom SS. Comparative investigations of the effects of Kanwa (Trona) and tokansenyi (plant potash) on liver and kidney of albino rats. International Journal of Biological and Chemical Sciences. 2018; 12(1):422-30.

Odiraa. Potash (Akanwu) consumption and the effects on Human Health. 2020.

Oladele FC, Airaodion AI, Agunbiade AP, Adedeji AA, Megwas AU, Ayita EB, et al. Hepatotoxic nature of potash (Kaun) in wistar rats. International Research Journal of Gastroenterology and Hepatology. 2021;4(2):40-51.

Ibeme IU. Medicinal Uses of Kanwa (Akanwu) in Nigeria. Healthy Living. Northeast Star Online Journal; 2015.

Javed M, Usmani N. Impact of heavy metal toxicity on hematology and glycogen status of fish: A review. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences. 2015; 85(4):889-900.

Loi F, Pilo G, Franzoni G, Re R, Fusi F, Bertocchi L, et al. Welfare assessment: Correspondence analysis of welfare score and hematological and biochemical profiles of dairy cows in Sardinia, Italy. Animals. 2021;11(3):854.

Iweka FK, Dic-Ijiewere OE, Oaikhena F, Bankole JK, Festus OO, Dada FL. The effect of potash on liver function of wistar rats. International Journal of Basic, Applied and Innovative Research. 2016;5(1):13-20.

Ogundare SO, Sirajuddin M, Iyevhobu KO, Ogundare EI. Histopathological effects of potash on the hearts of adult wistar rats. Asian Journal of Cardiology Research. 2020;6(3):23-30.

Ajayi AF, Akhigbe RE. Antispermatogenic mechanism of Trona is associated with lipid peroxidation but not testosterone suppression. J Hum Reprod Sci. 2017; 10(2):124-7.

Chinedu E, Arome D, Ameh FS. A new method for determining acute toxicity in animal models. Toxicology international. 2013;20(3):224.

Lorke D. A new approach to practical acute toxicity testing. Archives of toxicology. 1983;54(4):275-87.

Chinko BC, Amah-Tariah FS, Ekenna IC. Evaluation of the effects of calabash chalk on the haematological profile of wistar rats. Notulae Scientia Biologicae. 2022;14(3): 11281.

Evbu I, Precious O, Iriagbonse E. Haematological indices and blood lipid profile of rats administered Trona; A natural food additive. The Egyptian Journal of Biochemistry and Molecular Biology. 2016;34(1-2):15-22.

Olisa OG, Ajibade OM, Nafiu LO, Olajide-Kayode JO, Abdus-Salam MO. Mineralogy and health risk assessment of trona consumed in parts of Ibadan, southwestern Nigeria. Arabian Journal of Geosciences. 2022;15(15):1-13.

Uzoho CU, Onyekonwu M, Akaranta O. Characterization of local Alkali, surfactant and polymer used for enhanced oil recovery. International Journal of Engineering Research And Management (IJERM). 2019;6(5):41-6.

Ashour TH, El-Shemi AG. Caffeic acid phenyl ester prevents cadmium intoxication induced disturbances in erythrocyte indices and blood coagulability, hepatorenal dysfunction and oxidative stress in rats. Acta Haematologica Polonica. 2014;45(3):272-8.

Ovie K-S, Ikomi U. Alterations in some haematological parameters of the African snakehead: Parachanna Africans exposed to cadmium. Notulae Scientia Biologicae. 2011;3(4):29-34.

Von Garnier C, Stünitz H, Decker M, Battegay E, Zeller A. Pica and refractory iron deficiency anaemia: A case report. Journal of medical case reports. 2008;2(1):1-3.

Lanzkowsky P. Manual of pediatric hematology and oncology: Elsevier; 2005.

Kauffmann T, Evans DS. Macrocytosis. StatPearls [Internet]: StatPearls Publishing; 2021.

Lanzkowsky P. Classification and diagnosis of anemia in children. Lanzkowsky's Manual of Pediatric Hematology and Oncology: Elsevier. 2016:32-41.

Ani CO, Olasunkanmi MJ, Ene CB, Chime PU, Onwuka CO, Egharevba JE, et al. Assessment of the effects of fresh palm oil, natron (kanwa) and natron-treated palm oil on some hematological parameters in wistar rats. Journal of Medical Science and Clinical Research. 2017;5(6):24113-20.

Mahurpawar M. Effects of heavy metals on human health. Int J Res Granthaalayah. 2015;530:1-7.

Witeska M. Stress in fish-hematological and immunological effects of heavy metals. Electronic Journal of Ichthyology. 2005;1(1):35-41.

Blumenreich MS. The white blood cell and differential count. Clinical Methods: The History, Physical, and Laboratory Examinations. 3rd edition; 1990.

Gowda S, Desai PB, Hull VV, Avinash AK, Vernekar SN, Kulkarni SS. A review on laboratory liver function tests. The Pan African Medical Journal. 2009;3.

Macpherson I, Abeysekera KWM, Harris R, Mansour D, McPherson S, Rowe I, et al. Identification of liver disease: Why and how. Frontline Gastroenterology. 2022;3(5).

Hall P, Cash J. What is the real function of the liver ‘function’tests? The Ulster Medical Journal. 2012;81(1):30.

Hoekstra LT, de Graaf W, Nibourg GAA, Heger M, Bennink RJ, Stieger B, et al. Physiological and biochemical basis of clinical liver function tests: A review. Annals of surgery. 2013;257(1):27-36.

Hann H-W, Wan S, Myers RE, Hann RS, Xing J, Chen B, et al. Comprehensive analysis of common serum liver enzymes as prospective predictors of hepatocellular carcinoma in HBV patients. Plos One. 2012;7(10):e47687.

US Department of Health Human Services. Toxicological profile for cadmium. Draft for public comment; 1991.

Imam TS, Sani A, Hassan A. Heavy metals in blood and histopathological analysis of wistar rats exposed to five natron (kanwa) varieties sold in Kurmi Market Kano, Northern Nigeria. Fudma Journal of Science. 2019;3(3):472-81.

Gowda S, Desai PB, Kulkarni SS, Hull VV, Math AA, Vernekar SN. Markers of renal function tests. North American Journal of Medical Sciences. 2010;2(4):170.

Cholongitas E, Shusang V, Marelli L, Nair D, Thomas M, Patch D, et al. renal function assessment in cirrhosis–difficulties and alternative measurements. Alimentary pharmacology & therapeutics. 2007;26(7): 969-78.

Bankole JK, Ngokere AA, Ajibade OM, Igunbor CM, Eloka CCV. Degenerating effects of potash (Kaun-K2CO3) on the kidney: Unabated continental challenge to human health in Nigeria. Annals of Biology Research. 2015;6(3):12-8.