Mineral Content and Antisickling Activity of Annona senegalensis, Alchornea cordifolia and Vigna unguiculata Used in the Management of Sickle Cell Disease in the Kwilu Province (Congo, DR)

Main Article Content

Jules M. Kitadi
Clément L. Inkoto
Emmanuel M. Lengbiye
Damien S. T. Tshibangu
Dorothée D. Tshilanda
Koto-te-Nyiwa Ngbolua
K. M. Taba
Blaise M. Mbala
Brigitte Schmitz
Pius T. Mpiana


Aims: To determine the mineral composition of some plants (Annona senegalensis Pers., Alchornea cordifolia (Schumach. & Thonn.) Müll. Arg. and Vigna unguiculate (L.) Walp.) used in the management of sickle cell disease by traditional practitioners in Kwilu province and to evaluate their antisickling activity in vitro. 

Study Design: Plant collection in the Kwilu province, sample preparation,  antisickling tests and fluorescence spectrometric analysis.

Place and Duration of Study: This work was performed at the Faculty of Science, University of Kinshasa, Congo DR, from October 2016 to January 2018.

Methodology: These three plants were harvested in the province of Kwilu in Democratic Republic of the Congo. The mineral composition analysis was carried out using the fluorescence spectrometric method while the in vitro antisickling activity was evaluate using Emmel and hemolysis tests.

Results: Twenty three mineral elements were identified in each of these three plants: Potassium (K), Phosphorus (P), Calcium (Ca), Sodium (Na), Magnesium (Mg), Sulphur (S), Chlorine (Cl) and trace elements as: Aluminum (Al), Silicon (Si), Vanadium (V), Chromium (Cr), Manganese (Mn), Iron (Fe), Nickel (Ni), Copper (Cu), Zinc (Zn), Selenium (Se), Brome (Br), Molybdenum (Mo), Tin (Sn), Iodine (I), Barium (Ba) and Lead (Pb). Annona senegalensis Pers., Alchornea cordifolia (Schumach. & Thonn.) Müll.Arg. and Vigna unguiculate (L.) Walp. aqueous extracts showed the capacity to prevent the sickling and the hemolysis of red blood cells.

Conclusion: The obtained results confirm the antisickling activity thus justifying the use of these plants in Traditional Medicine for the management of sickle cell disease. The presence of some mineral elements like Fe, Zn, Mg and Se are useful for sickle cell disease patients.

Annona senegalensis, Alchornea cordifolia, Vigna unguiculata, sickle cell disease, mineral elements, fluorescence, antisickling

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How to Cite
Kitadi, J. M., Inkoto, C. L., Lengbiye, E. M., Tshibangu, D. S. T., Tshilanda, D. D., Ngbolua, K.- te-N., Taba, K. M., Mbala, B. M., Schmitz, B., & Mpiana, P. T. (2020). Mineral Content and Antisickling Activity of Annona senegalensis, Alchornea cordifolia and Vigna unguiculata Used in the Management of Sickle Cell Disease in the Kwilu Province (Congo, DR). International Blood Research & Reviews, 11(3), 18-27. https://doi.org/10.9734/ibrr/2020/v11i330131
Original Research Article


Mpiana PT, Makelele LK, Oleko RW, Bokota MT, Tshibangu DS, Ngbolua KN, Mbala MB, Atibu EK, Nsimba SM. Antisickling activity of medicinal plants used in the management of Sickle Cell Disease in Tshopo district, D.R. Congo, Australian Journal of Medical Herbalism. 2010;22(4):132-137.

Newinger HD. African traditional medicine. 1st Edn., Mephaim Scientific Publisher Stuttgart ; 2000.

Ngbolua KN, Mpiana PT, Mudogo V, Ngombe NK, Tshibangu DST, Ekutsu E, Kabena ON, Gbolo BZ, Muanyishay CL. Library search, Ethno-pharmacological survey and floristical study of some medicinal plants traditionally used to treat infectious and parasitic pathologies in the democratic Republic of Congo, The journal of medicinal plants, Photon. 2014;106:454-467.

WHO, traditional medicine strategy ; 2014-2023.


Accessed 05 August 2020.

Bishola TT, Ngbolua KN, Masengo CA, Gbolo BZ, Bongo GN, Tshibangu DST, Tshilanda DD, Mudogo V, Mpiana PT. Antibacterial activity of three validated antisickling plant species from the great apes pharmacopoeia in Congo-Kinshasa international. Journal of Biomedical and Clinical Sciences. 2020;5(1):33-40

Kehinde MO, Jaja SI, Adewumi OM, Adeniyi AI, Nezianya MO, Ayinla EO. Liver enzymes and trace elements in the acute phase of sickle cell anaemia Enzymes hépatiques et des éléments traces dans la phase aiguë de l’anémie à hématies falciformes West African Journal of Medicine. 2010;29(4):244-247

Bruneton, J. Pharmacognosie : Phytochimie des plantes médicinales, 5ème Ed. Lavoisier, Paris, 2016.

Mpiana PT, Misakabu FS, Tshibangu DST, Ngbolua KN, Mwanangombo DT. Antisickling activity and membrane stabilizing effect of anthocyanins extract from andansonia digitata L. Barks on sickle blood cells. International Blood Research & Review. 2014;(5):198-212.

Bongo G, Inkoto C, Masengo C, Tshiama C, Lengbiye E, Djolu R, Mutwale K, Kabamba K, Mbemba T, Tshilanda D, Mpiana PT, Ngbolua KN. Assessment of antisickling, antioxidant and antibacterial activities of some Congolese Taxa: Aframomum alboviolaceum (Ridley) K. Schum, Annona senegalensis Pers. and Mondia Whitei (Hook. f.) Skeels. American Journal of Laboratory Medicine. 2017;2(4): 52-59.

DOI: 10.11648/j.ajlm.20170204.13

Mpiana PT, Ngbolua KN, Atibu EK, Kasonga TM, Bokota MT, Mudogo V. In vitro effects of anthocyanins extracts from Justicia secunda VAHL on the solubility of hemoglobin S and membrane stability of sickle erythrocytes. Blood transfusion. 2010;8:248-254.

Tshilanda DD, Onyamboko DNV, Babady-Bila P, Ngbolua KN, Tshibangu DST, Dibwe EDF, Mpiana PT. Anti-sickling Activity of Ursolic Acid Isolated from the Leaves of Ocimum gratissimum L. (Lamiaceae) Nat. Prod. Bioprospect; 2015.

DOI : 10.1007/ s13659-015-0070-6

Muya JT, Mwanangombo DT, Tsalu PV, Mpiana PT, Tshibangu DST, Chung H. Conceptual DFT study of the chemical reactivity of four natural products with anti‑sickling activity Spinger Nature applied Sciences. 2019;1:1457.

Available:https ://doi.org/10.1007/s4245 2-019-1438-8

Adetutu RA, Kolawole N, Lateef A, Sulaimon, Bathlomew B, Ijaola A. Alterations of antioxidant status and mitochondrial succinate dehydrogenase activity in the liver of wistar strain albino rats treated with by ethanol extracts of Annona senegalensis pers (Annonaceae) Stem Bark. Toxicological Research. 2019; 35(1):13-24.


Osadebe PO, Okoye FBC. Anti-inflammatory effects of crude methanolic extractand fractions of Alchornea cordifolia leaves. Journal of Ethnopharmacology. 2003;89:19–24.

Mpiana PT, Ngbolua KN, Tshibangu DST. Les Alicaments et la drepanocytose : une mini revue. Comptes Rendus Chimie. 2016;19:884-889.


Ngbolua KN, Mpiana PT, Tshibangu DST, Mazasa PP, Gbolo BZ, Atibu EK, Kadima JN, Kasali, F. M. 2014. In vitro antisickling and radical scavenging activities of a poly-herbal formula (Drepanoalpha®) in sickle cell erythrocyte and acute toxicity study in Wistar albino rats. European Journal of Medicinal Plants. 2014;4(10):1251-1267.

Mpiana PT, Mudogo V, Tshibangu DST, Ngbolua KN, Atibu EK, Mbala BM, Tshilanda DD, Dianzenza E, Kakule MK. Antisickling properties, thermal and photochemical degradation of the anthocyanins extracted of Annona senegalensis from D R Congo. Int. J. Biol. Chem. Sci. 2012;6(5):2241-2251.

Kitadi JM, Mazasa PP, Tshibangu DST, Memvanga PB, Ngbolua KN, Taba NK, Noki PV and Mpiana PT. Anti-sickling and antioxidant activities of anthocyanins extracts from Dissotis brazzae Cogn (Melastomataceae) J. of Advancement in Medical and Life Sciences. 2015;V3I4.

DOI: 10.15297/ JALS.V3I4.01

Mpiana PT. Biophysique Médicale. Resud Edition, Tome I, 2ème Edition revisée, Kinshasa; 2015.

Kitadi JM, Inkoto CL, Lengbiye EM, Tshibangu DST, Tshilanda DD, Ngbolua KN, Taba KM, Mbala BM, Schmitz B, Mpiana PT. Antisickling activity and mineral content of Hura crepitans, Alternanthera bettzickiana and Dissotis brazzae, plants used in the management of sickle cell disease in kwilu province Democratic Republic of the Congo. European journal of pharmaceutical And medical research. 2019;6(12):79-83.

Daniel VN, Daniang IE, Nimyel ND. Phytochemical analysis and mineral elements composition of ocimum basilicum obtained in jos metropolis, Plateau state, Nigeria. International Journal of Engineering & Technology IJET-IJENS. 2011;11(06):135-137.

Ebenyi LN, Uraku AJ. Phytochemical and nutritional evaluation of aqueous and ethyl acetate leaf extracts of Alchornea cordifolia. Research Journal of Pharmacology and Pharmacy. 2017; 1(5):2572-8377.

Yoka J, Loumeto JJ, Gaudentius JD, Akouango P, Epron D. Evaluation des teneurs en éléments minéraux des feuilles de Niébé cultivés dans la zone Boundji, République du Congo. Journal of Applied Biosciences. 2014;79:6799-6807.

Brugnara C, De Franceschi L, Alper SL. Inhibition of Ca2+-dependent K+ transport and cell dehydration in sickle erythrocytes by CLT and other imidazole derivatives. J Clin Invest. 1993;92:520-526.

Franceschi L, Bachir D, Galacteros F, Tchernia G, Cynober T, Neuberg D, et al. Oral magnesium pidolate: effects of long‐term administration in patients with sickle cell disease. British Journal of Haematology. 2000;108(2):284‐289.

Martinez-Ballesta MC, Dominguez-Perles R, Moreno DA, Muries B, Alcaraz-Lopez C, Bastias E, Garcia-Viguera C, Carvajal M. Minerals in plant food: effect of agricultural practices and role in human health. A review Agron. Sustain. Dev. 2010;30:295–309.

DOI: 10.1051/agro/2009022

Agoreyo FO, Nwanze N. Plasma sodium and potassium changes in sickle cell patients. International Journal of Genetics and Molecular Biology. 2020; 2(2):014-019.

Mpiana PT, Ngbolua KN, Bokota MT, Kasonga KT, Atibu KE, Tshibangu DST, Mudogo V. In vitro effects of anthocyanin extract from Justicia secunda Vahl on the solubility of haemoglobin S and membrane stability of sickle erythrocytes. Blood Transfusion. 2010;8:248-54.

DOI: 10.2450/2009.0120-09

Kunle OF, Egharevba HO. Chemical constituents and biological activity of medicinal plants used for the management of sickle cell disease - A review. Journal of Medicinal Plants Research. 2013;7(48): 3452-3476.

DOI: 10.5897/JMPR2013.5333x

Adeboye HA, Tai CC, Qianli M, Lillian M, Jeff M, Alan M, Martin HS, Michael FH. Sickle cell bone disease: Response to vitamin D and calcium. American Journal of Hematology. 2008;3:271–274.

Rana YS, Tiwari OP, Krishan R, Sharma CM. Determination of nutritional potential of five important wild edible fruits traditionally used in Western Himalaya. Int. J. of Life Sci. 2018;6(1):79-86.

Mbemba F. Aliments et denrées alimentaires traditionnels du Bandundu en RD Congo : Répertoire et composition en nutriments, Ed L’Harmattan, Paris, 2013.

Palmer BF. Regulation of potassium homeostasis. Clin. J. Am. Soc. Nephrol. 2015;10:1050–1060.

Elinge CM, Muhammad A, Atiku FA, Itodo AU, Peni IJ, Sanni OM, Mbongo AN. Proximate, mineral and anti-nutrient composition of pumpkin (Cucurbita pepo L.) seeds extract. Int. J. Plant. Res. 2012; 2:146–150.

Al-Farga A, Zhang H, Siddeeg A, Shamoon M, Chamba MV, Al-Hajj N. Proximate composition, functional properties, amino acid, mineral and vitamin contents of a novel food: alhydwan (Boerhavia elegana Choisy) seed flour. Food Chem. 2016;211:268–273.

Sungu JK, Mukuku O, Mutombo AM, Mawaw P, Aloni MN, Luboya ON. Trace elements in children suffering from sickle cell anemia: A case–control study. J Clin Lab Anal. 2018;32:e22160.


Itoua OY, Elenga M., Moutsamboté J M, Mananga V., Mbemba F. Évaluation de la consommation et de la composition nutritionnelle des légumes-feuilles de Phytolacca dodecandra L’Herit consommés par les populations originaires des districts d’Owando et de Makoua. Journal of Animal & Plant Sciences. 2015; 27(1):4207-4218

Gupta A, Role of Zinc, Copper and Selenium in Nutritional Anemia. Nutritional anemia in preschool children. 2017 ;185-199.

Semba RD, Ferrucci L, Cappola AR, Ricks MO, Ray AL, Xue QL, Guralnik JM Linda PF Low Serum Selenium Is Associated with Anemia Among Older Women Living in the Community: The Women’s Health and Aging Studies I and II Biol Trace Elem Res. 2006;112(2):97-107.

DOI: 10.1385/BTER:112:2:97