Open Access Original Research Article

JAK-2V617F Mutation in Acute Leukemia (South Egypt Experience)

Hosny Badrawy, Abeer Ibrahim

International Blood Research & Reviews, Page 1-7
DOI: 10.9734/IBRR/2014/5340

Aim: To search for JAK2V617F mutation in patients with acute myeloid and acute lymphoblastic leukemia in south Egypt.

Study Design: JAK2V617F mutation detected by amplification refractory mutation system (ARMS) -PCR.

Place and Duration of Study: Department of clinical pathology and department of medical oncology, South Egypt Cancer Institute, Assiut University, Assiut, Egypt, between December  2010 and December 2012.

Method: We included 90 patients (58 men and 32 women; age range 2-67 years) with denovo acute leukemia (30 acute myeloid leukemia and 60 acute lymphoblastic leukemia), JAK-2 V617F mutation using   ARMS-PCR was done for all the patients.

Results: JAK-2 V617F mutation was absent in all of the studied patients.

Conclusion: Our results confirm the finding published previously which reported that JAK2 V617F mutation is very rare or absent in acute leukemia.


Open Access Original Research Article

Iron Status and Red Cell Parameters in Pregnant and Non-pregnant Adolescents in Côte d’Ivoire (West Africa)

Mathieu Nahounou Bleyere, Anthelme Semi Néné-Bi, Mama Kone, Duni Sawadogo, Paul Angoué Yapo

International Blood Research & Reviews, Page 8-22
DOI: 10.9734/IBRR/2014/5112

Aims: The objective of this study was to evaluate iron metabolism and compare iron stores between pregnant and non-pregnant adolescents in Côte d’Ivoire.

Place and Duration of Study: The study was undertaken with 187 volunteers adolescents aged from 15 to 19 years. For this study, adolescents were divided into 2 groups with 75 non-pregnant adolescents and 112 pregnant adolescents. Study population was recruited January 2006 to January 2008 in 4 urban community health centers, of Abidjan. Assays of blood samples were performed in Laboratory of Physiology, Pharmacology and Phytotherapy (Nangui Abrogoua University) and in Laboratory of Medical Biochemistry of University Hospital Centre (Cocody, Félix Houphouët-Boigny University).

Methodology: In each pregnant adolescent a blood sample was collected by venipuncture on a dry and EDTA tubes of 5 ml. With these blood samples, haematological and biochemical parameters were determined.

Results: Haematological parameters were decreased in pregnant adolescents at the third trimester of pregnancy compared with control adolescents. Pregnant adolescents were more anaemic during pregnancy (77.7 %) compared with non-pregnant adolescents (42.7 %). Iron stores were greatly decreased in 72.3 %, 83.9 % and 95.6 % of adolescents respectively during the 3 trimesters of pregnancy compared with non-pregnant adolescents (34.7 %). In addition, high prevalence of iron deficiency anaemia was recorded throughout pregnancy in adolescents. Therefore, iron status was more altered in pregnant adolescents (11.6 %, 9.8 % and 0.0 % respectively) compared with non-pregnant adolescents (13.3 %). The results of this study show that the causes of pronounced degradation in iron status are insufficient in size of iron stores in pregnant adolescents and non-pregnant adolescents.

Conclusion: Iron metabolism alteration is important in pregnant adolescents in Côte d’Ivoire, causing severe anaemia in this group of population.

Open Access Original Research Article

Comparison Effect of Moringa oleifera Leaf Meal and Oxytetracycline on Haematology and Serum Biochemical Profile of Broiler Finishers

A. D. Ologhobo, I. O. Adejumo, E. I. Akangbe

International Blood Research & Reviews, Page 29-36
DOI: 10.9734/IBRR/2014/6079

Aims: The study was carried out to assess the effects of Moringa oleifera leaf meal on the haematological parameters and serum biochemical profile of broiler finishers in comparison with oxytetracycline. 

Study Design:  The design of the study was a completely randomised design.

Place and Duration of Study: The study was carried out at the Poultry Unit of the Teaching and Research Farm, University of Ibadan, Nigeria. The study lasted for four weeks.  

Methodology: One hundred and eight 4 weeks old un-sexed arbor acre broiler chicks were used for the study. Four dietary treatments containing 3064.27-3073.55 Kcal/kg of energy and 19.75% -21.94% crude protein were formulated and each treatment had three replicates with nine birds per replicate. The experimental diets contained 250g of oxytetracycline per 100kg of feed for treatment 1 (T1), 200g, 400g and 600g of Moringa oleifera leaf meal per 100kg of feed for treatments 2 (T2), 3 (T3) and 4 (T4) respectively.

Results: The results of the study revealed that there was no significant difference across the treatments for most of the parameters measured. Red blood cell (RBC) counts for birds fed T4 (4.70 x106/mm3) were significantly (P<0.05) higher than those fed the control diet (3.61 x106/mm3). Birds fed T4 recorded highest aspartate amino transferase (AST) mean value (72.18%) which was significantly (P<0.05) higher than the mean value of those fed the control diet T1 (52.26%).

Conclusion: It can be concluded from the result of the study that Moringa oleifera leaf meal could be used as alternative antibiotic in place of oxytetracycline. The result of the study suggested that Moringa oleifera leaf has antimicrobial properties.


Open Access Original Research Article

Prostate Specific Antigen (PSA) Screening among Apparently Healthy Men of African Descent in Sokoto, North Western, Nigeria

O. Erhabor, Isaac Zama, A. S. Mainasara, R. A. Shehu, I. P. Iwueke, Aghedo Festus, D. Ikhuenbor, E. K. Uko, O. O. Igbineweka

International Blood Research & Reviews, Page 37-47
DOI: 10.9734/IBRR/2014/6362

Background: Globally prostate cancer is the sixth leading cause of cancer-related death in men. Prostate Specific Antigen (PSA) is present in small amount in the serum of men with healthy prostates, but is often elevated in the presence of prostate cancer and other prostate-related disorders. The aim of this present study was to determine the PSA levels among healthy men of African descent resident in Sokoto, North Western Nigeria.

Methods: Testing was carried out using the CTK Biotech PSA kit (CTK Biotech Inc, San Diego, USA). The Onsite PSA Rapid Test is a lateral flow chromatographic immunoassay for the qualitative detection of prostate specific antigen (PSA) in human serum or plasma at a cut-off level of 4.0 ng/mL.

Result: We investigated the PSA levels in 106 consecutively recruited men of African descent with age range and mean age of 40-70 years and 44.75 ± 7.91 years respectively. We observed a PSA of > 4ng/mL among 7 (6.6%) of subjects studied. Men > 60 had the highest prevalence of raised PSA level (33.3%) compared to those younger men (p= 0.001). We observed a higher prevalence among farmers (11.1%) compared to business men, civil servants and students.

Conclusion: In this present study we observed a high prevalence of PSA >4ng/mL and a positive and significant correlation between age and raised PSA levels among men in Sokoto, North Western Nigeria. We recommend an annual PSA blood test for men ≥ 40 years of age and that those with a PSA value > 4.0 ng/mL should be considered for further evaluation. There is also the need to build capacity among biomedical and medical staff in Nigeria to facilitate effective diagnosis of prostate cancer. There is also the need for increased prostate cancer awareness in the area to facilitate voluntary testing.


Open Access Case Study

Pure Red Cell Aplasia in a Patient with Cri-Du-Chat

Jing Ai, Valeria Visconte, Heesun J. Rogers, Mikkael A. Sekeres, Ramon V. Tiu

International Blood Research & Reviews, Page 23-28
DOI: 10.9734/IBRR/2014/6978

Background: The Cri-Du-Chat syndrome also known as 5p- syndrome is a rare genetic autosomal disorder with the characteristic deletion of the short arm (p) of chromosome 5.  To date, hematologic manifestations characteristic of BM failure have not been linked to this syndrome. 

Aims: To evaluate the safety and efficacy of cyclosporine to treat pure red cell aplasia in the clinical setting of 5p- syndrome. 

Presentation of Case: We report here a patient with 5p- syndrome who subsequently developed idiopathic pure red cell aplasia. He was treated with cyclosporine 1mg/kg twice a day, and achieved a complete remission, with no toxicities. 

Conclusion: This case suggests that cyclosporine can be used safely and effectively in such clinical scenario.