Open Access Original Research Article

The Frequency and Distribution of ABO Blood Groups in Patients with Haematological Cancers in Uyo, Nigeria: A Hospital Based Retrospective Study

Mabel Benson Ino-Ekanem, Timothy Amos Ekwere, Anyiekere Morgan Ekanem

International Blood Research & Reviews, Page 1-8
DOI: 10.9734/IBRR/2018/42665

Background: The pattern and distribution of haematological malignancies vary depending on age, sex and geographical location. Studies on the association between haematological cancers and ABO blood types have been largely conflicting.

Aim: To determine the frequency and distribution of ABO blood groups among a cohort of patients with haematological cancers.

Methods: This was a retrospective descriptive cross-sectional study involving the review of records of patients with various haematological cancers, their demographic characteristics and ABO blood group distribution over a 5 year period.

Results: The record of 132 patients with haematological malignancies over a 5 year period were reviewed. The mean age of the patients was 47.93 ± 17.9 years. The peak age incidence for the various haematological cancer was the fourth and fifth decades of life. The male to female sex ratio was 2:1. NHL was the commonest haematological cancer among the patients. Majority of the patients were of the O-blood type, the different blood group distribution among the patients was statistically not different from that of the general population. Also, there was no significant association between haematological cancers and ABO blood type of the patients.

Conclusion: The distribution, age and sex ratio of haematological malignancies in our study was comparable to those reported by other authors with NHL being the most common haematological cancer in our environment. Majority of the patients were of the O-blood type, comparable to the general population. There was no significant association between haematological cancers and ABO blood type of the patients.


Open Access Original Research Article

Seroprevalence of Salmonella Agglutinins among Apparently Healthy Students of a Tertiary Institution in North-Eastern Nigeria

M. Y. Tula, O. Iyoha, R. O. Okojie, J. Filgona, G. A. Onyeje

International Blood Research & Reviews, Page 1-7
DOI: 10.9734/IBRR/2018/43362

Aim: To determine the level of Salmonella agglutinin and its titre among apparently healthy students of Tertiary Institution in North-eastern Nigeria.

Study Design: A cross-sectional study on Seroprevalence of Salmonella agglutinin;

Place and Duration of the Study: Department of Biological Science Technology, Federal Polytechnic Mubi, Adamawa State, between September to December, 2016.

Methodology: This study was a cross-sectional study in which 200 apparently healthy  students of Federal Polytechnic Mubi were tested for Salmonella agglutinin using both slide and tube agglutination methods.

Statistical Analysis: Non-parametric Mann-Whitney statistics and least significance difference (LSD) were used to test for significant difference in all the data obtained. Significance difference was taken when P˂0.05.

Results: The result showed that the number of male with positive Widal agglutinin was significantly higher than that of female (P=0.028). The number of positive titre was significantly higher in age group 21-25 years (P=0.002) when compared to other age brackets. However, there was no statistical difference between the age bracket 16-20 years and 31-35 years (P=0.747). The result of the agglutination titre showed that more male had Salmonella titre for Salmonella Typhi O (81.8%) and Salmonella Typhi H (72.7%) while more female had Salmonella agglutinin titre for Salmonella Typhi H (44.4%) and Salmonella Paratyphi B-H (33.3%). It was also observed that only agglutinin for Salmonella Paratyphi A-O, B-O, C-O, B-H and C-H were present in the sera of male subjects up to the titre of 160 and at frequencies ranging from 1.6-58.8%. Furthermore, among females, it was observed that only agglutinins for Salmonella Paratyphi A-O, Salmonella Typhi C-H and Salmonella Typhi O and H were present in the sera of the subjects up to the titre of 160 and at frequencies ranging from 2.5-25.0%.

Conclusion: Detecting Salmonella agglutinin titre up to 160 among apparently healthy individuals is of public health significance. Thus, there is need to monitor this trend and create awareness on environmental and behavioural risk factors for Salmonella infections.


Open Access Original Research Article

Impact of DNMT3A Gene Mutation on Response of Acute Myeloid Leukemia Patients to Induction Therapy

Dina G. Segai, Nevene Ramsis, Hanaa Fahmy, Noha Kamel

International Blood Research & Reviews, Page 1-8
DOI: 10.9734/IBRR/2018/44030

Aim: To evaluate the frequency and prognostic impact of DNA methyltransferase 3A (DNMT3A) gene mutation on response to induction therapy in newly diagnosed acute myeloid leukemia patients.

Study Design: Cross-sectional descriptive study.

Place and Duration: Hematology units of Suez Canal and Ain Shams schools of Medicine, Egypt. Between September 2016 and July 2017.

Methodology: The study enrolled forty patients (male: female ratio was 1; mean age was 52.4 ± 19.4 years) with newly diagnosed de novo AML before starting induction therapy. DNMT3A mutations were detected using dye terminator sequencing technique for the second part of DNMT3A, encompassing the PHD and methyltransferase domains and representing exons 11 till the last exon 23. Hematological, cytogenetic studies and DNMT3A mutation results were compared to the patients’ hematological response to induction therapy.

Results: Fourteen patients (35%) of the study participants had DNMT3A mutations while 65% had the wild type. Approximately 49.5% of mutations occurred in exon 23, the most common mutations were (R882C and R882H mutations; 28.5% and 21%, respectively). Out of 14 patients with DNMT3A mutation, 9 patients had incomplete remission and only 5 achieved complete remission with no statistically significant association. Odds ratio of the response to induction therapy according to DNMT3A mutation status was 1.32 times higher to show incomplete remission than in wild-DNMT3A patients.

Conclusion: DNMT3A mutation has high prevalence in AML Egyptian patients with non-statistically significant difference between mutated DNMT3A and wild type when related to the impact on remission rates after induction therapy.


Open Access Original Research Article

Hematological Assessment of Benzene Exposure among Employees in Brega Oil Marketing Company (BOMC), Benghazi

Huda Mohamed, Maha A. Swani, Majdeddin. I. Alaghib, Abdeljawad I. Abdeljawad, Murad Alkezza, Mohamed Alobaidy

International Blood Research & Reviews, Page 1-7
DOI: 10.9734/IBRR/2018/43266

Background:  Benzene is a toxic chemical and is a human carcinogenic. It is found in both the natural and industrial atmosphere. Its exposure can cause hematological health problems as a result of metabolite formation in the liver and bone marrow. The aim of this study is to evaluate the hematological health effects of benzene exposure amongst workers in loading and uploading stations at the Ras-Elmengar depository of BOMC, Benghazi, Libya.

Methods:  This study was a cross sectional design with the study sample being 30 participants, 15 exposed workers and 15 non-exposed employees. They were asked to fill out a questionnaire about socio-demographic data and symptoms of benzene exposure. Blood samples were also taken from the participants by a laboratory technician to test the complete blood counts. The data from the blood test was analyzed using SPSS.

Results: The findings demonstrated a significant difference in CBC between the exposed and non-exposed groups. There was a reduction in WBC, RBCs and HCT of workers who were exposed to benzene. Moreover, there was a positive correlation between the hematological effects of benzene exposure and the BMI, years of experience and smoking habits of the workers.

Conclusion: The exposure to benzene has negative effects on the workers blood, and this effect is associated with work duration. Furthermore, it correlates with BMI and the smoking habits of the workers. Therefore, safety measures must be applied to protect the employees working at the company.

Open Access Minireview Article

FLT3 Mutations: Significance in Paediatric AML

Salma M. Aldalal

International Blood Research & Reviews, Page 1-7
DOI: 10.9734/IBRR/2018/42466

Patients with acute myeloid leukaemia (AML) who have FMS-like tyrosine kinase 3 (FLT3) mutations are a concern for haematologists. Many studies indicate that these patients have poor prognosis. Due to the dearth of research in this area, the role of allogeneic transplantation as a therapy is still controversial. But the available limited data suggest that transplantation in first remission is possibly the first choice. As FTL3 mutations in AML result in poor patient outcomes, different FLT3 inhibitors are used as specific targeted therapy.